The aimed of current research to formulate tooth gel utilizing leaf extract of Aloe Vera. In multiple clinical studies and used in dentistry for wound healing effect, gingivitis, plaque control and curing oral mucosal lesions. Aloe Vera is natural, ancient ingredient. The formulated Aloe Vera tooth gel evaluated by physical examination: Colour- Yellowish brown, Appearance-Homogeneous, Smooth nature, Transparency-Translucent and Relative density10.5, No microbial growth in sample plate, pH-7.5, Viscosity-3100cp, Extrudability amount percent-91.33, Spreadability- 6.5cm/sec and observed good stability. The anti- microbial evaluation against S.aureus reveal the formulated Aloe vera tooth gel exhibited notable activity with ZOI of 19.5 mm at MIC of 25µg/mL. The outcome of this research herbal tooth gel shows equal patronizing and engrossing passion over the marketed preparation it was consider after the comparing the marketed preparation (Colgate, Dabour Red, Dant-kanti, Close up) with formulated herbal tooth gel. It has good scope in future dental research and dental health of public.